In vitro mature embryo culture protocol of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat under boron stress |
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Authors: | A??l Ferdi Örgeç Mehmet Karaka? Fatma Pehlivan Verma Sandeep Kumar Zencirci Nusret |
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Institution: | 1.Department of Biology, Faculty of Science and Art, Bolu Abant ?zzet Baysal University, G?lk?y, 14200, Bolu, Turkey ;2.Institute of Biological Science, SAGE University, Kailod Kartal, Rau Bypass Road, 452020, Indore, Madhya Pradhesh, India ; |
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Abstract: | Mature embryos of einkorn (Triticum monococcum ssp. monococcum) and bread (Triticum aestivum L.) wheat were used for callus induction on media containing four different doses (0, 1, 2 and 4 mg L?1) of 2,4-D and dicamba supplemented with five different boron concentrations (0, 6.2, 12.4, 24.8, and 37.2 mg L?1). The obtained callus was transferred to culture media with three (0, 0.5, and 2 mg L?1) different BAP doses with five boron concentrations for further regeneration. The maximum callus weight in einkorn wheat was in culture media with 1 mg L?1 dicamba and 6.2 mg L?1 (3.71?±?0.13 g). Bread wheat had the maximum callus weight on culture media with 4 mg L?1 dicamba and 12.4 mg L?1 (3.46?±?0.40 g). The highest plantlet numbers were in only 2 mg L?1 BAP (2.92?±?0.88) for einkorn wheat and 0.5 mg L?1 BAP supplemented with 6.2 mg L?1 boron (3.71?±?1.12) for bread wheat. This indirect regeneration protocol using mature embryos of einkorn and bread wheat under boron stresses expected to be useful for future wheat breeding studies. |
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