Accuracy of calculated free testosterone differs between equations and depends on gender and SHBG concentration |
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| Authors: | Jennifer S. Hackbarth Jonathan B. Hoyne Stefan K. Grebe Ravinder J. Singh |
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| Affiliation: | 1. Department of Internal Medicine II, Charles University, Pilsen, Czech Republic;2. Biomedical Centre, Faculty of Medicine in Pilsen, Charles University, Czech Republic;3. Laboratory of Genetics, Charles University, Pilsen, Czech Republic;1. Department of Psychology, Humboldt-Universität zu Berlin, Berlin, Germany;2. Behavioral Epidemiology, Institute of Clinical Psychology and Psychotherapy, Technische Universität Dresden, Dresden, Germany;3. Institute of Clinical Chemistry and Laboratory Medicine, University Medicine Greifswald, Greifswald, Germany;4. School of Public Health and Preventive Medicine, Monash University, Melbourne, Australia;5. Department of Psychiatry and Psychotherapy, University Medicine Greifswald, Greifswald, Germany;6. Institute for Community Medicine, University Medicine Greifswald, Greifswald, Germany;7. German Centre for Cardiovascular Research, Partner Site Greifswald, University Medicine Greifswald, Greifswald, Germany;8. Department of Physiological and Clinical Psychology, University of Greifswald, Greifswald, Germany |
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| Abstract: | Serum free testosterone (fT) concentrations are often calculated, however different equations often yield discrepant results. This study explores the sources of this variability. We compared three established and two new equations that differed only by their testosterone association constants with isotope dilution equilibrium dialysis in two patient groups with different gender distributions. Equation components were examined to determine how they impacted correlation with isotope dilution equilibrium dialysis. Association constants derived for each patient group correlated best with isotope dilution equilibrium dialysis for that group and not the other set. Samples with the poorest correlation between isotope dilution equilibrium dialysis and calculated fT results had significantly higher SHBG concentrations. Regardless of equation, ≥25% of samples showed unacceptable deviation from isotope dilution equilibrium dialysis. Association constants and gender makeup and SHBG concentration of the patient groups used to establish an equation all significantly impact correlation with isotope dilution equilibrium dialysis. Application of many fT equations to wider populations will therefore frequently yield results that differ substantially from isotope dilution equilibrium dialysis. |
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