A novel in vitro flow system for changing flow direction on endothelial cells |
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Authors: | Wang Chong Lu Hao Schwartz Martin Alexander |
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Affiliation: | Robert M. Berne Cardiovascular Research Center, University of Virginia, Charlottesville, VA 22908, USA. |
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Abstract: | Atherosclerotic plaques localize to regions of flow disturbance, i.e. bifurcations, branch points and regions of high curvature. Shear stress in these regions can be multi-directional due to complex flow patterns such as time-varying vortices. However, commonly used in vitro flow models are incapable of changing flow orientation to any direction other than the reverse. We have developed a novel in vitro flow system to enable changes in flow direction to any angle. When cells were pre-aligned in laminar shear, and then rotated 90°, cells re-aligned over 24 h. Re-alignment involved actin remodeling by gradual rotation of actin stress fibers. This device will enable that analysis of how endothelial cells sense changes in flow direction as occur in vivo. |
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