Plasmid-controlled variation in the content of methylated bases in single-stranded DNA bacteriophages M13 and fd |
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Authors: | S Hattman |
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Institution: | University of Rochester Department of Biology Rochester, N.Y. 14627, U.S.A. |
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Abstract: | The N6-methyladenine and 5-methylcytosine contents in the DNA of bacteriophages M13 and fd have been analyzed. The results are summarized as follows. (1) After growth in bacteria harboring the N-3ft? drug resistance-factor, fd and M13 are observed to contain approximately 1 to 2 more 5-methylcytosine residues per DNA molecule than after growth in the parental drug-sensitive host; no effect on the N6-methyladenine content is produced by the plasmid. (2) After growth in bacteria harboring P1 prophage, fd and M13 are observed to contain approximately 2 to 3 more N6-methyladenine residues per DNA molecule than after growth in the parental P1-sensitive host; no apparent effect on the 5-methylcytosine content was produced by the P1 plasmid. (3) In agreement with others, fd carrying B-host specificity (fd·B) is observed to contain 2 more N6-methyladenine residues/DNA molecule than fd·K. |
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