| 乳链菌肽前体基因（nisZ）在乳酸乳球菌中的克隆和表达 |
| |
| 引用本文： | 陈秀珠,胡海菁,杨巍,还连栋. 乳链菌肽前体基因（nisZ）在乳酸乳球菌中的克隆和表达[J]. 遗传学报, 2001, 28(3): 285-290 |
| |
| 作者姓名： | 陈秀珠 胡海菁 杨巍 还连栋 |
| |
| 作者单位： | 中国科学院微生物研究所微生物资源前期开发国家重点实验室，北京 100080 |
| |
| 基金项目： | 国家自然科学基金(39570008)、国家“九五”科技攻关(96-C03-01-05)和中国科学院重点研究项目 |
| |
| 摘 要： | 用PCR技术从克隆有完整乳链菌肽生物合成基因簇（来自于乳链菌肽高产菌株L.lactis AL2)的重组噬菌体λHJ-3中扩增了编码乳链菌肽的前体基因，与pMG36e连接得到重组质粒pHJ201，用电击转化法将pHJ201转化到L.lactis NZ9800中，经活性测定和Tricine－SDS－PAGE电泳证实乳链菌肽前体基因获得了功能表达。DNA序列分析表明乳链菌肽高产菌株L.lactis AL2产生的是NisinZ。发现pHJ201d L.lactis NZ9800 中有良好的稳定性。
|
| 关 键 词： | 乳酸乳球菌 乳链菌肽前体基因 基因克隆 表达 |
| 文章编号： | 0379-4172(2001)03-0285-06 |
| 修稿时间： | 2000-07-12 |
Cloning and Expression ofnisZ Gene in Lactococcus lactis |
| |
| Abstract: | The gene encoding the precursor of nisin was amplified by PCRusing the λHJ-3 DNA as the template, which contained the entire nisin biosynthesis gene cluster from Lactococcus lactis AL2 with high yield of nisin, and was cloned into pMG36e. The recombinant plasmid Phj201 was introduced into Lactococcus lactis NZ9800 by electroporation. Phj201 is very stable in L. Lactis NZ9800. Antimicrobial activity test and Tricine-SDS-PAGE analysis revealed that L. Lactis NZ9800 harbouring Phj201 restored ability of nisin production, but the production level was markedly lower than L. Lactis AL2. The result of DNA sequence analysis indicated that Nisin Z is produced by L. Lactis AL2. |
| |
| Keywords: | Lactococcus lactis nisZ gene gene cloning expression |
| 本文献已被 维普 万方数据 等数据库收录！ |
