| 脑啡肽增强胶质细胞的神经营养作用与NO生成减少有关 |
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| 作者姓名： | Wei GW Du LN Zhu CQ Tang CR Cao XD Wu GC |
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| 作者单位： | 上海医科大学神经生物学教研室,医学神经生物学国家重点实验室,上海,200032 |
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| 基金项目： | 国家教委博士点基金,国家自然科学基金 |
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| 摘 要： | 本文在ＳＤ大鼠大脑皮层胶质细胞神经元共培养模式上,以神经元存活、突起生长、生长相关蛋白４３（ｇｒｏｗｔｈａｓｏｃｉａｔｅｄｐｒｏｔｅｉｎ４３,ＧＡＰ４３）ｍＲＮＡ的表达为指标,观察了脑啡肽对胶质细胞神经营养作用的影响,并对其机理作了初步探讨。结果表明,经脑啡肽处理的胶质细胞能使神经元的存活计数增加２８％（Ｐ＜００５）,单个神经元突起总长度增加１１％（Ｐ＜００５）,最长突起长度增加１６％（Ｐ＜００５）,ＧＡＰ４３ｍＲＮＡ的表达增加２６％（Ｐ＜００５）。然后又观察了脑啡肽（１０－６～１０－１２ｍｏｌ／Ｌ）对培养胶质细胞生成一氧化氮（ＮＯ）的影响。结果表明,浓度为１０－８,１０－１０ｍｏｌ／Ｌ的脑啡肽能明显抑制其生成（Ｐ＜００５）。结果提示,脑啡肽可能增强胶质细胞的神经营养作用,其机制之一可能是通过抑制胶质细胞ＮＯ的生成。
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| 关 键 词： | 脑啡肽 胶质细胞-神经元共培养 GAP-43 NO |
Effect of enkephalin on glial neurotrophic function related to the reduction of nitric oxide production |
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| Wei GW,Du LN,Zhu CQ,Tang CR,Cao XD,Wu GC.Effect of enkephalin on glial neurotrophic function related to the reduction of nitric oxide production[J].Acta Physiologica Sinica,1999,51(3):327-332. |
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| Authors: | Wei G W Du L N Zhu C Q Tang C R Cao X D Wu G C |
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| Institution: | State Key Laboratory of Medical Neurobiology, Department of Neurobiology, Shanghai Medical University, Shanghai 200032. |
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| Abstract: | The effect of methionine enkephalin (ME) on glial neurotrophic function was studied in rat cortical glial neuronal coculture. The results showed that ME treated glia enhanced neuronal survival by 28% ( P <0.05), and increased total neurite length per neuron by 11% ( P <0.05), while the expression of GAP 43 mRNA by 26% ( P <0 05). The effect of ME on NO production of glia was also studied in rat cortical glial culture. The result showed that different concentrations of ME (10 -12 , 10 -10 , 10 -8 , 10 -6 mol/L) inhibited glial NO production. All the above show that the increase of glial neurotrophic ability by ME may be due to the inhibition of glial NO production. |
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| Keywords: | methionine enkephalin glial neuronal coculture GAP 43 NO |
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