| 提高大肠杆菌通过MVA途径合成异戊二烯 |
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| 引用本文： | 冯凡,许杨,陶勇,刘伟丰,林白雪. 提高大肠杆菌通过MVA途径合成异戊二烯[J]. 生物工程学报, 2015, 31(7): 1073-1081 |
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| 作者姓名： | 冯凡 许杨 陶勇 刘伟丰 林白雪 |
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| 作者单位： | 1 南昌大学食品科学与技术国家重点实验室，江西 南昌 330047；2 南昌大学中德联合研究院，江西 南昌 330047,1 南昌大学食品科学与技术国家重点实验室，江西 南昌 330047；2 南昌大学中德联合研究院，江西 南昌 330047,3 中国科学院微生物研究所，北京 100101,3 中国科学院微生物研究所，北京 100101,3 中国科学院微生物研究所，北京 100101 |
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| 基金项目： | 中国科学院重点部署项目 (No. KGZD-EW-606-2)，国家重点基础研究发展计划 (973计划) (No. 2012CB721105) 资助。 |
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| 摘 要： | 异戊二烯是橡胶合成的重要前体物质。为了提高菌株的异戊二烯产量,本实验室在研究中构建了一株异戊二烯产气的菌株BW-01,基于蛋白质预算理论的指导,理性设计通过改变质粒拷贝数、增加稀有密码子等合成生物学手段调控关键限速酶编码基因表达,从而提高大肠杆菌外源MVA代谢途径的异戊二烯产量。摇瓶发酵实验中我们构建的新产气菌株BW-07比原有的产气菌株BW-01的产量提高了73%,达到了761.1 mg/L。为后续菌株改造及进行发酵罐实验奠定了基础。
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| 关 键 词： | 蛋白质预算，MVA途径，异戊二烯，大肠杆菌 |
| 收稿时间： | 2015-01-07 |
Improving isoprene production by engineered heterologous mevalonate pathway in Escherichia coli |
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| Fan Feng,Yang Xu,Yong Tao,Weifeng Liu and Baixue Lin. Improving isoprene production by engineered heterologous mevalonate pathway in Escherichia coli[J]. Chinese journal of biotechnology, 2015, 31(7): 1073-1081 |
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| Authors: | Fan Feng Yang Xu Yong Tao Weifeng Liu Baixue Lin |
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| Affiliation: | 1 State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China; 2 Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, Jiangxi, China,1 State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, Jiangxi, China; 2 Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, Jiangxi, China,3 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China,3 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China and 3 Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China |
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| Abstract: | Isoprene is an important precursor of synthetic rubber material. In our previous study, metabolic engineered Escherichia coli strain (BW-01) was constructed and used to produce isoprene. Based on the theory of protein budget, using synthetic biology strategies including the increased copy number of genes and rare codons, we regulated the expression of key enzyme to improve isoprene production in Escherichia coli strain. Under shake-flask conditions, isoprene productivity of the engineered strain (BW-07) increased by 73% compared with BW-01, reached 761.1 mg/L. It provides a reference for further studies. |
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| Keywords: | protein budget mevalonate pathway isoprene Escherichia coli |
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