| 小麦NBS-LRR类抗病基因同源cDNA序列的分离与鉴定 |
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| 引用本文： | 张立荣,杨文香,刘大群. 小麦NBS-LRR类抗病基因同源cDNA序列的分离与鉴定[J]. 植物遗传资源学报, 2011, 12(3): 431-436 |
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| 作者姓名： | 张立荣 杨文香 刘大群 |
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| 作者单位： | 河北农业大学植物保护学院植物病理系/河北省农作物病虫害生物防治工程技术研究中心,保定,071001 |
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| 摘 要： | 利用抗病基因的保守结构设计引物,从抗叶锈病近等基因系材料TcLr24中扩增出一条703bp的条带RGAl,通过与GenBank比对,选取与RGAI高度同源的若干条带,在它们共有的保守序列位置设计引物,利用cDNA末端快速扩增(RACE):ffL术扩增抗病同源基因cDNA全长.扩增到3条全长cDNA,经BLASTp比较,这些序列都舍有NBS保守结构域和多个LRR结构域.与很多已知植物抗病基因的功能相应区域一致.对FRGA-1,、FRGA-2和FRGA-3实时定量PCR分析,表明这3个基因在小麦叶片中都是组成型表达.本研究在小麦材料TcLr24中得到3条抗病基因同源cDNA全长,为研究小麦抗病基因奠定了基础.
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| 关 键 词： | 小麦抗叶锈病基因 eDNA末端快速扩增技术 生物信息学 同源基因 |
| 收稿时间： | 2010-09-24 |
| 修稿时间： | 2011-03-05 |
Cloning and character of resistance homologenes contained NBS-LRR in wheat TcLr24 |
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| ZHANG Li-rong,YANG Wen-xiang,LIU Da-qun. Cloning and character of resistance homologenes contained NBS-LRR in wheat TcLr24[J]. Journal of Plant Genetic Resources, 2011, 12(3): 431-436 |
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| Authors: | ZHANG Li-rong YANG Wen-xiang LIU Da-qun |
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| Affiliation: | ZHANG Li-rong,YANG Wen-xiang,LIU Da-qun(Biological Control Center of Plant Diseases and Pests of Hebei Province/College of Plant Protection,Agricultural University of Hebei,Baoding 071001) |
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| Abstract: | Resistance gene cDNA homology sequence from wheat was isolated using homology-based method.The primers were designed according to the conserved sequence of resistance gene analogs and a 703bp fragment was isolated from TcLr24.A few homologous sequences were captured when processing similarity search in GenBank database.We designed primers based on highly conserved region among the above sequences and the rapid amplification cDNA ends(RACE)was used to obtain the full length sequence of disease resistance hom... |
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| Keywords: | wheat leaf rust resistance gene rapid amplification cDNA ends (RACE) bioinformatics homologenes |
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