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| 甘草花粉超微鉴定及花粉活力、柱头可授性荧光显微镜观察 | |
| 引用本文： | 马春英,屈平,王文全. 甘草花粉超微鉴定及花粉活力、柱头可授性荧光显微镜观察[J]. 植物遗传资源学报, 2011, 12(3): 396-401 |
| 作者姓名： | 马春英 屈平 王文全 |
| 作者单位： | 1. 河北农业大学农学院/河北省作物生长调控重点实验室,保定,071001 2. 河北农业大学生命学院,保定,071001 3. 北京中医药大学中药学院/中药材规范化生产教育部工程研究中心,北京,100102 |
| 基金项目： | 国家科技重大专项"重大新药创制"课题，河北省自然科学基金，河北省科技厅项目 |
| 摘 要： | 对甘草的有性生殖特性进行了研究,利用扫描电子显微镜对乌拉尔甘草、光果甘草和胀果甘草花粉的超微结构进行观察,利用荧光显微镜确定乌拉尔甘草的授粉方式、花粉生活力和柱头活性以及授粉后受精时间.结果表明,花粉超微结构为甘草的鉴别提供了一定的形态学依据;甘草的授粉方式属于闭花受精;花粉生活力在每天12:00时最强,去雄后超过4d的柱头已经不具备接受花粉的能力;对授粉后不同时间的雌蕊进行研究得出授粉后6h花粉管进入胚珠进行受精. |
| 关 键 词： | 甘草 花粉活力 柱头可受性 荧光显微镜 |
Identification of Pollen Ultrastructure and Fluorescence Microscope Observation of Pollen Viability and Stigma Acivity of Liquorice |
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| MA Chun-ying,QU Ping,WANG Wen-quan. Identification of Pollen Ultrastructure and Fluorescence Microscope Observation of Pollen Viability and Stigma Acivity of Liquorice[J]. Journal of Plant Genetic Resources, 2011, 12(3): 396-401 | |
| Authors: | MA Chun-ying QU Ping WANG Wen-quan |
| Affiliation: | MA Chun-ying1,QU Ping2,WANG Wen-quan3(1 Key Laboratory of Regulation and Control of Crop Growth of Hebei Province/College of Agronomy,Agricultural University of Hebei,Baoding 071001,2School of Life Science,3 Engineering Research Center for Chinese Medicine Standardized Production of Educational Ministry/School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 100102) |
| Abstract: | The ultrastructure of the pollen in the Glycyrrhiza uralensis Fisch.,G.glabra L.and G.inflata Batal.were observed by the scanning electron microscope(SEM).Pollination way,pollen viability stigma activity and the fertilization time ascertainment after pollination were observed by the fluorescence microscope.The results showed that the study on the ultrastructure of pollen offered certain morphology basis for the discrimination of the liquorice.The pollination way of the liquorice was cleistogamy.The pollen v... |
| Keywords: | Liquorice Pollen viability Stigma activity Fluorescence microscope |
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