| 青藏高原早熟甘蓝型春油菜遗传资源研究 |
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| 引用本文： | 张晓梅,姚艳梅,徐亮,杜德志. 青藏高原早熟甘蓝型春油菜遗传资源研究[J]. 植物遗传资源学报, 2011, 12(3) |
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| 作者姓名： | 张晓梅 姚艳梅 徐亮 杜德志 |
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| 作者单位： | 1. 青海大学农林科学院春油菜研究所/国家油菜改良青海分中心/青海省高原作物种质资源创新与利用重点实验室,西宁,810016
2. 青海大学农林科学院春油菜研究所/国家油菜改良青海分中心/青海省高原作物种质资源创新与利用重点实验室,西宁,810016;西宁市蔬菜研究所,西宁,810016 |
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| 基金项目： | 国家油菜产业技术体系，国家科技支撑计划 |
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| 摘 要： | 利用SSR和SRAP 2种分子标记研究了69份试验材料的遗传差异及其亲缘关系.29对SSR标记共扩增出118条多态性带,多态性位点占总扩增位点的97.5%,27对SRAP引物扩增出123条多态性带,多态性比率为70.3%.两种标记聚类结果表明.在相似系数0.566处所有材料可以分为A、B 2个大类群;B类在相似系数0.620处又可分为7个亚类,10个天然双低早熟甘蓝型品系、2个甘蓝型亲本和4个新型品系聚在第1亚类中,其余的51个新型甘蓝型油菜品系分别聚在其他6个亚类中.对55份新型品系进行遗传成分分析,结果表明,每个品系都合有4种带型,各品系所舍不同带所占比率不同.对各品系中含有白菜型亲本带所占比率分别与其对应的两亲本之间的遗传距离进行相关分析,结果表明新型甘蓝型油菜品系中白菜型亲本带所占比率与白菜型素本间的遗传距离为负相关(-0.52),且达到极显著水平;与甘蓝型亲本间的遗传距离为正相关(0.31),且达到显著水平.对试验材料之间的遗传距离及其来源进行分析(除与2个白菜型亲本间),遗传距离排名前20位的都来自新型品系之间或天然品系与新型之间,最大为0.544.
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| 关 键 词： | 遗传距离 甘蓝型油菜 |
Genetic Resources of Brassica napus L.Strains with Early Maturity in Qinghai-tibet Plateau |
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| ZHANG Xiao-mei,YAO Yan-mei,XU Liang,DU De-zhi. Genetic Resources of Brassica napus L.Strains with Early Maturity in Qinghai-tibet Plateau[J]. Journal of Plant Genetic Resources, 2011, 12(3) |
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| Authors: | ZHANG Xiao-mei YAO Yan-mei XU Liang DU De-zhi |
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| Affiliation: | ZHANG Xiao-mei1,YAO Yan-mei1,XU Liang1,DU De-zhi1,2(1Qinghai Plateau Germplasm Resources and Key Laboratories/Qinghai Sub-Center of National Rapeseed Improvement/Institute of Spring Rapeseed,Qinghai Academy of Agriculture and Forestry,Qinghai University,Xining 810016,2Xining Institute of Vegetable Research,Xining 810016) |
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| Abstract: | Genetic differenes and their relationships of 69 materials were studied by using SSR and SRAP molecular markers.29 SSR markers amplified 118 polymorphic bands,in which 97.5% were polymorphic.123 polymorphic bands were amplified with 27 SRAP primers,percentage of polymorphic loci was 70.3%.Cluster analysis indicated that all the materials can be grouped into 2 classes(A and B) when the similarity coefficient was 0.566.B class can be grouped into 7 subclasses when similarity coefficient was 0.620.10 nature bi... |
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| Keywords: | SSR SRAP |
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