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| 拟南芥中RING型E3泛素连接酶基因AtGW2的克隆和功能分析 | |
| 引用本文： | 李会勇.拟南芥中RING型E3泛素连接酶基因AtGW2的克隆和功能分析[J].植物遗传资源学报,2011,12(3):448-454. |
| 作者姓名： | 李会勇 |
| 作者单位： | 1. 黑龙江大学农作物研究院,哈尔滨,150080;河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002 2. 河南省农业科学院粮食作物研究所/河南省玉米生物学重点实验室,郑州,450002 3. 黑龙江大学农作物研究院,哈尔滨,150080 |
| 基金项目： | 国家转基因生物新品种培育重大专项 |
| 摘 要： | 水稻RING型E3泛素连接酶基因OsGW2在调控水稻产量性状方面起着十分重要的作用.根据OsGW2的cDNA序列,通过RT-PCR方法从拟南芥中克隆了一个与OsGW2同源的基因,命名为AtGW2.序列分析表明,该基因编码一个RING-C2型E3泛素连接酶蛋白,含有401个氨基酸.通过构建AtGW2 RNA干扰植物表达栽体并转化拟南芥,结果表明,获得的转基因后代植株的子粒较野生型大,并且转基因拟南芥子粒千粒重高于野生型,这表明AtGW2负调控拟南芥子粒大小及粒重. |
| 关 键 词： | 拟南芥 RING型E3泛素连接酶 粒重 |
| 收稿时间： | 2010/10/21 0:00:00 |
| 修稿时间： | 2011/1/24 0:00:00 |
Cloning and Functional Analysis of Arabidopsis thaliana AtGW2, a RING-type E3 Ubiquitin Ligase Protein |
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| lihuiyong.Cloning and Functional Analysis of Arabidopsis thaliana AtGW2, a RING-type E3 Ubiquitin Ligase Protein[J].Journal of Plant Genetic Resources,2011,12(3):448-454. | |
| Authors: | lihuiyong |
| Institution: | JIANG Ying1,2,CAO Yan-yong2,LU Yun-cai1,TANG Bao-jun2,WANG Li-feng2,LI Hui-yong2(1Institute for Crop Research,Heilongjiang University,Harbin 150080,2 Henan Provincial Key Laboratory for Maize Biology/Cereal Crop Research Institute,Henan Academy of Agricultural Sciences,Zhengzhou 450002) |
| Abstract: | OsGW2 (RING type E3 ubiquitin ligase) plays important roles in regulating rice yielding traits. According to the OsGW2 cDNA sequences, the homolog of OsGW2 was isolated from Arabidopsis thaliana by RT-PCR and designated as AtGW2. The predicted AtGW2 protein has 401 amino acids, and has a RING-type domain. The grain size and kilo kernels weight of wild-type Arabidopsis plants were lower than those of AtGW2 RNA interference transgenic plants. These results suggested that AtGW2 negatively regulates the grain size and weight in Arabidopsis. |
| Keywords: | AtGW2 RNAi |
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