Optimization of growth performance of freshly induced carrot suspensions concerning PMP production |
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Authors: | Heike Mikschofsky Martin Hammer Jörg Schmidtke Patricia König Günther Keil Horst Schirrmeier Kerstin Schmidt Inge Broer |
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Institution: | 1.Agrobiotechnologie,Universit?t Rostock,Rostock,Germany;2.Friedrich-Loeffler-Institut,Greifswald–Insel Riems,Germany;3.BioMath GmbH,Rostock,Germany |
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Abstract: | Carrot suspension cultures are efficient production systems for plant-made pharmaceuticals (PMP); however, frequently, the
reduction of transgene expression in long-running transgenic cell lines limits the productivity. Freshly induced carrot suspensions
have been identified for a constant and high biomass production with optimization of culture conditions and cultivars. A small-volume
monitoring method was adapted to freshly induced carrot suspension that minimized the heterogeneity of freshly induced meristematic
carrot cell lines. Using this system combined with the adaptation of growth conditions, we identified the cultivar Rote Riesen
as having the highest increase in dry biomass with an average of 6.5 g/L weekly, stable and consistent after 8 wk. As a model
PMP, VP60—the capsid protein from the rabbit hemorrhagic disease virus—was produced in a carrot suspension with a maximal
accumulation of 2.4 μg/g dry mass. The developed method guarantees comparable investigations of different transgene expression
in various freshly induced small-volume carrot cell lines. |
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