Localization, morphology and function of the mitochondria-rich cells in relation to transepithelial Na(+)-transport in chicken lower intestine (coprodeum)

The correlation between morphology of the mitochondria-rich cells (MR cells) in chicken lower intestine, coprodeum, and dietary sodium levels, has been investigated, using hens with differing dietary intake of NaCl and plasma aldosterone levels. Additionally, the function of the MR cells was evaluated in relation to proton secretion/exchange. Epithelium from the coprodeum was examined by optical, transmission and scanning electron microscopy, and Na(+)-transport across the coprodeal epithelium was measured electrophysiologically in Ussing-chambers. To investigate the function of MR cells, lectin-, enzyme- and immunohistochemistry methods were used. The MR cells were generally located in the epithelium on the upper parts of the sides of mucosal folds. Long microvilli, high but variable toluidine blue affinity/electrondensity and numerous mitochondria were the main features distinguishing them from the surrounding epithelial cells. Two main MR cell types were observed, differing in microvillous morphology, diameter and toluidine blue affinity/electrondensity. This probably reflected differences in maturity and activity. The MR cells expressed a positive carbonic anhydrase reaction and a proton exchange similar to the absorptive intestinal epithelial cells, but exhibited no specific demonstrable proton secretion. A close correlation between the ultrastructure of the MR-cells, dietary sodium levels, plasma aldosterone and transepithelial Na-transport was observed.