Incorporation of anthraquinonyl group into lambda-Cro repressor protein for strand- and position-specific photocleavage of double-stranded DNA |
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Authors: | Sasaki Hiroshi Ikeda Kazumasa Suzuki Maya Ninomiya Keiko Sisido Masahiko |
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Institution: | Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University 3-1-1 Tsushimanaka, Okayama 700-8530, Japan. |
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Abstract: | lambda-Cro repressor protein incorporated with a 2-anthraquinonylalanine (anqAla) at the 64th position was chemically synthesized by solid-phase method. The 64th position was selected according to previous information on various mutants of Cro incorporated with a single anqAla unit, that were synthesized through an Escherichia coli in vitro protein-synthesizing system. The 64anqAla mutant bound to a dsDNA of consensus operator sequence and underwent strand- and position-specific photocleavage of the dsDNA at the GG sequence after treatment with piperidine. The mutant also underwent position-specific self-photoscission. The self-photoscission was retarded in the presence of the dsDNA. |
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