Semen characteristics and sperm morphology of serow (Capricornis sumatraensis) |
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Authors: | Suwanpugdee A Kornkeawrat K Saikhun K Siriaroonrat B Tipkantha W Doungsa-Ard K Sa-Ardrit M Suthunmapinatha P Pinyopummin A |
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Affiliation: | a Center of Agricultural Biotechnology, Kasetsart University, Nakhon Pathom 73140, Thailand b Faculty of Technology and Community Development, Thaksin University, Phattalung 93110, Thailand c Faculty of Veterinary Medicine, Kasetsart University, Nakhon Pathom 73140, Thailand d Institute of Science and Technology for Research and Development, Mahidol University, Nakhon Pathom 73140, Thailand e Department of Research and Conservation, Zoological Park Organization, Bangkok 10300, Thailand f The Zoological Park Organization, Changmai 30000, Thailand g Laboratory Animal Center, Mahidol University, Nakhon Pathom 73140, Thailand |
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Abstract: | The serow (Capricornis sumatraensis) is a critically endangered species. The objectives of this study were to evaluate ejaculate quality in captive males, and to investigate and characterize sperm morphology. Semen was collected using electroejaculation. Mean (±S.D.) seminal characteristics were: semen volume 2.3 ± 0.8 mL, pH 7.8 ± 0.4, and osmolality 329.9 ± 32.9 mOsmol/kg; sperm concentration 515.8 ± 263.1 × 106 cells/mL; wave motion score (1-5) 3.9 ± 0.4; motile sperm 60.5 ± 22%; viable sperm 68.3 ± 9.4%; morphologically normal sperm 70.8 ± 19.3%; and an opacity that was yellowish to milky-white. Sperm head length, width, degree of elongation, area, and perimeter were 6.0 ± 0.6 μm, 4.3 ± 0.3 μm, 71.7 ± 8.6%, 19.8 ± 2.5 μm2, and 17.9 ± 2.1 μm. Based on these measurements, we categorized sperm head morphometry as small, medium, or large. In addition, sperm morphology was examined by light and scanning electron microscopy; overall, morphologically normal and abnormal sperm were similar to those reported for other bovidae. In summary, this study provided baseline data regarding semen characteristics of C. sumatraensis, which should be of value in the preservation of this endangered species. |
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Keywords: | Serow Capricornis sumatraensis Semen Sperm morphology |
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