Morphological and cytological diversity of regenerants derived from half-anther cultures of anthurium |
| |
Authors: | Budi Winarto Fitri Rachmawati Dewi Pramanik Jaime A Teixeira da Silva |
| |
Institution: | (1) Indonesian Ornamental Crops Research Institute, Jl. Raya Ciherang, Pacet-Cianjur, 43253, West Java, Indonesia;(2) Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki-cho, Ikenobe 2393, Kagawa-ken 761-0795, Japan |
| |
Abstract: | Anthurium anther culture was successfully established using half-anthers as explants. Explants were cultured on Winarto–Teixeira basal
medium (WT-1) containing 0.01 mg/l α-naphthalene acetic acid (NAA), 0.5 mg/l thidiazuron (TDZ), and 1.0 mg/l 6-benzylaminopurine
(BAP), or on New Winarto–Teixeira basal medium (NWT-3) supplemented with 0.02 mg/l NAA, 1.5 mg/l TDZ, and 0.75 mg/l BAP for
callus initiation. Regenerated calli produced multiple shoots on WT-1, which were then rooted in NWT-3 supplemented with 1%
activated charcoal. Plantlets were acclimatized ex vitro using a mixture of burned rice husk, rice husk, and bamboo peat (1:1:1,
v/v/v) as the potting medium. There was considerable morphological and cytological diversity of regenerants derived from anther
culture, which are described in detail in this study. The callus cluster color ranged from green to light green and had a
high regeneration capacity (7.3 and 4.8 shoots/callus cluster), light reddish-yellow callus showed moderate regeneration (2.6
shoots/callus cluster), while reddish-yellow callus had the lowest regeneration capacity (1.5 shoots/callus cluster). Morphological
variations clearly observed in regenerants derived from this technique included alterations in plant size, peduncle length,
spathe position compared to leaves, the type and number of buds, spathe and spadix color, and spadix length. There were also
cytological variations in both in vitro and ex vitro regenerants of anther culture with 23–29% haploids, 5–10% aneuploids,
56–69% diploids, and 3–4% triploids. The results strengthen other studies in which the development of anther cultures, especially
via callus formation, resulted in morphological and cytological alterations. These variations have been discussed to great
length in this paper. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|