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Extracellular production of a glycolipid biosurfactant, mannosylerythritol lipid, from Candida antarctica
Authors:Hee-Sik Kim  Jong-Woon Jeon  Hong-Won Lee  Yong-Il Park  Weon-Taek Seo  Hee-Mock Oh  Tohoru Katsuragi  Yoshiki Tani  Byung-Dae Yoon
Affiliation:(1) Korea Research Institute of Bioscience and Biotechnology, Oeun-dong 52, Yusong, Taejon, 305-333, Korea;(2) Department of Food Processing, Chinju National University, Chilam-dong, Chinju, 660-758, Korea;(3) Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma, Nara, 630-01, Japan
Abstract:Candida antarctica (sp. SY16) required avegetable oil as the carbon source to produce a biosurfactant, mannosylerythritol lipid (MEL-SY16). Biosurfactant production was 31 g l–1 after 7 days in a batch culture and was not growth associated. In a two-stage culture, glycerol and oleic acid were used as an initial and a feeding carbon source, respectively, and 41 g l–1 biosurfactant was produced after 8 days.
Keywords:biosurfactant  Candida sp.  mannosylerythritol lipid  production  two-stage culture
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