Membrane cholesterol content accounts for developmental differences in surface B cell receptor compartmentalization and signaling |
| |
Authors: | Karnell Fredrick G Brezski Randall J King Leslie B Silverman Michael A Monroe John G |
| |
Institution: | Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6160, USA. |
| |
Abstract: | Recent studies argue for an important role for cholesterol in maintaining plasma membrane heterogeneity and influencing a variety of cellular processes, including signaling, adhesion, and permeability. Here, we document that tolerance-sensitive transitional immature B cells maintain significantly lower membrane unesterified cholesterol levels than mature-stage splenic B cells. In addition, the relatively low level of cholesterol in transitional immature B cells impairs compartmentalization of their B cell receptor (BCR) into cholesterol-enriched domains following BCR aggregation and reduces their ability to sustain certain aspects of BCR signaling as compared with mature B cells. These studies establish an unexpected difference in the lipid composition of peripheral transitional immature and mature B cells and point to a determining role for development-associated differences in cholesterol content for the differential responses of these B cells to BCR engagement. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|