Production of erythriod cells from human embryonic stem cells by fetal liver cell extract treatment |
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Authors: | Yu-xiao Liu Wen Yue Lei Ji Xue Nan Xue-tao Pei |
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Institution: | (1) Stem Cell and Regenerative Medicine Lab, Beijing Institution of Transfusion Medicine, Beijing, 100850, China;(2) Department of Neurosurgery, the First Affiliated Hospital of PLA General Hospital, Beijing, 100037, China |
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Abstract: | Background We recently developed a new method to induce human stem cells (hESCs) differentiation into hematopoietic progenitors by cell
extract treatment. Here, we report an efficient strategy to generate erythroid progenitors from hESCs using cell extract from
human fetal liver tissue (hFLT) with cytokines. Human embryoid bodies (hEBs) obtained of human H1 hESCs were treated with
cell extract from hFLT and co-cultured with human fetal liver stromal cells (hFLSCs) feeder to induce hematopoietic cells.
After the 11 days of treatment, hEBs were isolated and transplanted into liquid medium with hematopoietic cytokines for erythroid
differentiation. Characteristics of the erythroid cells were analyzed by flow cytometry, Wright-Giemsa staining, real-time
RT-PCR and related functional assays. |
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