A New Model of Vaginal Infection by <Emphasis Type="Italic">Candida albicans</Emphasis> in Rats |
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Authors: | Márcia A Carrara Lucélia Donatti Edílson Damke Terezinha I E Svidizinski Márcia E L Consolaro Márcia R Batista |
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Institution: | 1.Department of Clinical Analyses,State University of Maringá,Maringá,Brazil;2.Department of Cell Biology,Federal University of Curitiba,Curitiba,Brazil |
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Abstract: | Vulvovaginal candidiasis (VVC) is regarded as an important public health issue, and several aspects of its pathogenesis are
not yet sufficiently clear. Experimental in vivo models of vaginal infection with Candida albicans have been extremely useful in the identification of factors concerning hormonal influences on the infection, the virulence
of the yeasts, the susceptibility, and the treatment of the infection. The development of easily manageable, reproducible,
and economically viable animal models of VVC is highly important. We describe a simple experimental model of VVC in rats,
using a pharmaceutical brand of estradiol hexa-hydrobenzoate for human treatment. All the steps of this model were standardized;
and after the experiments, the rats were euthanized for further examination of their tissues by scanning and transmission
electron microscopy. Standardized features included the use of non-ovariectomized rats, sterile distilled water as the hormone
vehicle, estradiol hexa-hydrobenzoate administered at 0.20 mg/week/rat fractionated three times/week, and a yeast suspension
of 5 × 108 yeasts/ml in a single vaginal administration 1 week after hormone induction. In this way, 100% of the rats were in pseudo-estrus
and developed and maintained the infection until the third week of the experiment. Electron microscopy observation of the
vagina of the rats confirmed the presence of both pseudo-estrus and vaginal infection. The standardized experimental model
proved inexpensive, reproducible, and easily feasible for the induction of vaginal infection with C. albicans and may help to clarify important aspects of the pathogenesis and treatment of VVC. |
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