Temporin/VesCP (T/V)-like antibiotic peptides, derived from frogs and wasps, induce migration of human monocytes and neutrophils |
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Authors: | De Yang Qian Chen Joost J Oppenheim Pentti Kuusela John W Taylor and David Wade |
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Institution: | (1) Department of Molecular and Integrative Physiology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;(2) Department of Chemistry, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA;(3) Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; |
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Abstract: | Summary Several naturally occurring antimicrobial peptides, from mammals and insects, have previously been shown to be chemotactic
for human inflammatory cells. Based on this evidence, ten synthetic analogs of naturally occurring antibiotic peptides from
the skin secretions of three species of Ranid frogs and the venom of one species of Vespid wasp (i.e., T/V-like peptides)
were tested for their abilities to induce migration of human neutrophils and monocytes. These included temporin A (TA fromRana temporaria), temporin 1P (T1P fromR. pipens), ranateurin 6 (Rana-6 fromR. catesbeiana)], three TA analogs all D-amino acids (D-TA), reversed sequence (Rev-TA), and Pro3→Gly (G3-TA)], two frog skin-related T/V-like
peptide consensus sequences (I4S10-Con and I4G10-Con), VesCP-M (VCP-M fromVespa mandarinia), and a hybrid peptide composed of portions of the insect antibiotic peptide, cecropin A (CA), and TA (CATA). TA, T1P, Rana-6,
VCP-M, G3-TA, I4S10-Con, I4G10-Con, and CATA all induced cell migration at micromolar concentrations. D-TA and Rev-TA did
not induce cell migration, suggesting that this process involves a chiral interaction, such as receptor binding, and also
depends on the order of amino acids within TA. The results demonstrate, for the first time, that certain T/V-like antibiotic
peptides are capable of inducing chemotaxis of human phagocytes and suggest that these peptides are multifunctional molecules
with antimicrobial, hemolytic, and chemotactic capabilities. |
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Keywords: | chemotaxis consensus sequence D-amino acid frog monocyte MRSA neutrophil temporin VRE wasp |
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