| 艾氏腹水癌细胞核糖核酸体外对C_(57)BL/6脾自然杀伤活性的影响 |
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| 引用本文： | 肖国芝,董苍玉,王丹.艾氏腹水癌细胞核糖核酸体外对C_(57)BL/6脾自然杀伤活性的影响[J].中国生物化学与分子生物学报,1991,7(1):117-121. |
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| 作者姓名： | 肖国芝 董苍玉 王丹 |
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| 作者单位： | 北京医科大学生物化学教研室，北京医科大学生物化学教研室，北京医科大学生物化学教研室 北京 100083，北京 100083，北京 100083 |
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| 摘 要： | 本文采用~(51)Cr释放法,研究了艾氏腹水癌细胞核糖核酸(EAC-RNA)体外对C_(57)BL/6小鼠脾自然杀伤细胞(NK)对YAC-1靶细胞杀伤活性的影响。结果表明,EAC-RNA能显著抑制NK活性。经RNase处理后,其抑制活性消失,DNase或Pronase的处理不改变其抑制活性。
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| 关 键 词： | 艾氏腹水癌核糖核酸 肿瘤核糖核酸 ~(51)Cr释放法 自然杀伤活性 |
| 收稿时间： | 1991-02-20 |
Influence of EAC-RNA on C_(57)BL/6 Spleen Natural Killer Activity in Vitro |
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| Xiao,Guo-zhi Dong,Cang-yu Wang,Dan.Influence of EAC-RNA on C_(57)BL/6 Spleen Natural Killer Activity in Vitro[J].Chinese Journal of Biochemistry and Molecular Biology,1991,7(1):117-121. |
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| Authors: | Xiao Guo-zhi Dong Cang-yu Wang Dan |
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| Institution: | (Department of Biochemistry, Beijing Medical University, Beijing 100083 |
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| Abstract: | EAC-RNA and normal RNA(N-RNA)were obtained from Ehrilich Ascite cancer cells and normal murine liver respectively, and then incubated in vitro with effect cells. In comparison with N-RNA, EAC-RNA can markedly inhibite the NK activity of effect cells against YAC-1 target cells. This inhibitory effect disappeared when EAC-RNA was treated with RNase. Treatment of DNase or Pronase did not affect the activity of EAC-RNA. From these experiments, we suggest that tumor RNA may be considered as one of the causes of the NK activity inhibition in tumor host. |
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| Keywords: | EAC-RNA Tumor RNA 51Cr-release assay Natural Killer activity |
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