Analysis of genetically modified plant gene expression using GUS fluorimetry |
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| Authors: | Kevan M A Gartland Angela T McHugh Stanislav Vitha Karel Benes Richard J Irvine Jill S Gartland |
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| Institution: | (1) Plant Biotechnology Research Group, Molecular & Life Sciences, University of Abertay Dundee, DD1 1HG, Scotland;(2) Institute of Plant Molecular Biology & South Bohemia University, Ceske Budejovice, Czech Republic |
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| Abstract: | A fluorimetric assay method for the analysis of beta-glucuronidase (GUS) reporter gene expression in genetically modified
plants is described. Optimization of this method for woody plants and a statistical approach suitable for comparisons of gene
expression in different transformants or tissues of the same plant is described. Example data from elm (Ulmus procera) SR4 regenerant plants, shown to be genetically modified by PCR and DNA-DNA hybridizations, in which higher GUS expression
levels are found in stems than in leaves demonstrates the utility of this approach. |
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| Keywords: | β -Glucuronidase fluorimetry gene regulation reporter gene elm Ulmus procera promoters |
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