Thromboxane A2 stimulates mitogen activated protein kinase and arachidonic acid liberation in rabbit platelets |
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| Authors: | Satoko Ohkubo Norimichi Nakahata Yasushi Ohizumi |
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| Affiliation: | Department of Pharmaceutical Molecular Biology, Faculty of Pharmaceutical Sciences, Tohoku University Aoba, Aramaki, Aoba-ku, Sendai 980, Japan |
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| Abstract: | U46619, a thromboxane A2 mimetic, caused tyrosine phosphorylation of several proteins in rabbit platelets. Among them, 42 kDa protein was identified as a mitogen-activated protein kinase (MAPK). U46619 activated MAPK in a concentration-dependent manner, measured by incorporation of 32P to a specific substrate for MAPK. U46619 also liberated [3H)arachidonic acid in a concentration-dependent manner. The U46619-induced MAPK activation and [3H]arachidonic acid liberation were inhibited by SQ29548 and by the removal of external Ca2+ ions. This is a first demonstration that TXA2 activates MAPK accompanied with arachidonic acid liberation in rabbit platelets. |
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| Keywords: | thromboxane A2 tyrosine kinase mitogen-activated protein kinase arachidonic acid cytosolic phospholipase A2 |
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