Chromosomal localization of parsley 4-coumarate: CoA ligase genes by in situ hybridization with a complementary DNA |
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| Authors: | Pung-Ling Huang Klaus Hahlbrock Imre E. Somssich |
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| Affiliation: | (1) Max-Planck-Institut für Züchtungsforschung, D-5000 Köln 30, Federal Republic of Germany;(2) Present address: Plant Gene Expression Center, USDA - U.C. Berkeley, 94710 Albany, CA, USA |
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| Abstract: | A near full-length cDNA (1.9 kb) was used as probe forin situ hybridization to assign one of the two highly homologous 4-coumarate: CoA ligase genes in parsley (Petroselinum crispum) to the short arm of a submetacentric chromosome. The results suggest, but do not definitely prove, that the second gene is located on a metacentric chromosome and is thus unlinked from the other.Abbreviations CHS chalcone synthase - 4CL 4-coumarate:CoA ligase - Kb kilobases (kilobasepairs) |
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