A simple and rapid extraction of high molecular weight chromosomal DNA from Bacillus subtilis protoplasts for cosmid cloning and interspecific transformation |
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| Authors: | Akamatsu T Taguchi H Okada H |
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| Institution: | Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan. akamatsu@bio.kumamoto-it.ac.jp |
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| Abstract: | After conversion of Bacillus subtilis vegetative cells to protoplasts, a simple and rapid method for extracting high-molecular-weight chromosomal DNA was devised with the inclusion of bovine serum albumin and phenol-chloroform treatments. The DNA sample thus prepared was the size of 100-450 kb and could be used for cosmid cloning and interspecific transformation. |
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