Structural features and selective expression of three Ly-5+ cell-surface molecules |
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Authors: | Jwu-Sheng Tung Margrit P Scheid Marco A Pierotti Ulrich Hämmerling Edward A Boyse |
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Institution: | (1) Memorial Sloan-Kettering Cancer Center, 1275 York Ave., 10021 New York, New York;(2) Present address: Divisione Oncologia Sperimentale, Istituto Nazionale Tumori, Milano, Italy |
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Abstract: | Conventional Ly-5 alloantisera precipitate cell-surface molecules of three sizes: 200K, 205K, and 220K. In SDS-PAGE, the rat monoclonal antibody 74/8 precipitates the same three molecules from both Ly-5.1 and Ly-5.2 cells. Cleveland mapping of the three molecules, precipitated by reaction of conventional Ly-5 alloantisera or 74/8 monoclonal antibody with lysates of 125I-labeled cells, disclosed no differences among the three molecular forms, but markedly distinguished all three Ly-5.1 molecules from all three Ly-5.2 molecules. Each of the three molecular forms can be expressed independently of the other two by cloned culture lines of Ly-5+ cells of different hematopoietic lineage. All of the seven cloned lines tested expressed only one form. However, two of the seven uncloned culture lines tested, plasmacytoma MOPC-70A and the X.1 putative macrophage line which originated from an SJL tumor, yielded both 200K and 205K forms.American Cancer Society Research Professor of Cell Surface Immunogenetics |
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