Phosphorothioate G3T4G3 motifs inhibits the early stage of HIV-1 infection. |
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| Authors: | J Suzuki N Miyano-Kurosaki H Takeuchi Y Tamura G Kawai K Takai Y Tanaka R Tanaka N Yamamoto H Takaku |
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| Affiliation: | Department of Industrial Chemistry, Chiba Institute of Technology, Japan. |
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| Abstract: | It is well known that G-rich phosphorothioate oligonucleotides (G-rich PS) bind to the V3 loop of HIV-1 gp120 and inhibit HIV-1 infection. In this study, we investigated the inhibitory mechanism of a new type of G-rich PS (PS-G3T4G3) on the replication cycle of HIV-1. PS-G3T4G3 inhibits both cell to cell and cell free infections. Binding and entry assays revealed that the inhibitory step of PS-G3T4G3 occurs at the early stage of HIV-1 infection. V3 loop-specific mAb test showed that PS-G3T4G3 binds to the V3 loop and prevents its interaction with chemokine receptors. These results suggest that PS-G3T4G3 may be a novel candidate for an HIV-1 inhibitor. |
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