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Investigation of enzyme formulation on pretreated switchgrass |
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| Authors: | Falls Matthew Shi Jian Ebrik Mirvat A Redmond Tim Yang Bin Wyman Charles E Garlock Rebecca Balan Venkatesh Dale Bruce E Pallapolu V Ramesh Lee Y Y Kim Youngmi Mosier Nathan S Ladisch Michael R Hames Bonnie Thomas Steve Donohoe Bryon S Vinzant Todd B Elander Richard T Warner Ryan E Sierra-Ramirez Rocio Holtzapple Mark T |
| Affiliation: | a Texas A&M University, College Station, TX 77843, United States b University of California, Riverside, CA 95207, United States c Michigan State University, East Lansing, MI 48824, United States d Auburn University, Auburn, AL 36849, United States e Purdue University, West Lafayette, IN 47907, United States f Ceres, Inc., Thousand Oaks, CA 91320, United States g National Renewable Energy Laboratory, Golden, CO 80401, United States h Genencor, A Danisco Division, Palo Alto, CA 94304, United States i Universidad de los Andes, Grupo de Conversion de Energia, Bogotá, Colombia |
| Abstract: | This work studied the benefits of adding different enzyme cocktails (cellulase, xylanase, β-glucosidase) to pretreated switchgrass. Pretreatment methods included ammonia fiber expansion (AFEX), dilute-acid (DA), liquid hot water (LHW), lime, lime + ball-milling, soaking in aqueous ammonia (SAA), and sulfur dioxide (SO2). The compositions of the pretreated materials were analyzed and showed a strong correlation between initial xylan composition and the benefits of xylanase addition. Adding xylanase dramatically improved xylan yields for SAA (+8.4%) and AFEX (+6.3%), and showed negligible improvement (0-2%) for the pretreatments with low xylan content (dilute-acid, SO2). Xylanase addition also improved overall yields with lime + ball-milling and SO2 achieving the highest overall yields from pretreated biomass (98.3% and 93.2%, respectively). Lime + ball-milling obtained an enzymatic yield of 92.3 kg of sugar digested/kg of protein loaded. |
| Keywords: | Switchgrass Pretreatment Xylanase β-glucosidase Enzymatic digestion |
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