Proteomics to identify pathogenesis-related proteins in rice roots under water deficit |
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Authors: | Ling Yang Na Su Mancheng Wu Changchun Wang Haitao Hu |
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Institution: | (1) The State Key laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, 310029 Hangzhou, P. R. China;(2) Present address: College of Life Science, Zhejiang Forestry University, 311300 Lin An, P. R. China; |
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Abstract: | Upland and lowland rice (Oryza sativa L.) showed different mechanisms of water stress resistance. Hydroponically grown 3-week-old seedlings of a lowland variety
IR64 and an upland variety were exposed to 15% polyethylene glycol (PEG-6000). After 7 d of treatment, IR64 maintained high
relative water content and developed a well-branched root. Therefore, IR64 had better water-deficit tolerance than Azucena
under PEG treatment. To identify water-deficit-responsive proteins associated with the tolerance differences between two ecotypes,
a comparative proteomic analysis of roots was conducted. Out of 700 proteins reproducibly detected on two-dimensional electrophoresis
gels, 65 proteins exhibited significant changes in at least one ecotype at 48 h of water deficit. Only 15 proteins showed
different responses to water deficit between the two ecotypes. Twelve proteins were identified by matrix-assisted laser desorption/ionization-time
of flight/time of flight-mass spectrometry, which involved in energy and metabolism, protein processing and degradation, detoxification
and pathogenrelated (PR) proteins, i.e. PR-1a, RSOsPR10 and JIOsPR10. All three PR proteins were induced more strongly in
IR64 than in Azucena by water deficit at both protein and mRNA level. The results suggested that PR-1a, RSOsPR10 and JIOsPR10
may play important roles in protecting root cells against water deficit in rice. |
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