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Proteomics to identify pathogenesis-related proteins in rice roots under water deficit
Authors:Ling Yang  Na Su  Mancheng Wu  Changchun Wang  Haitao Hu
Institution:(1) The State Key laboratory of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, 310029 Hangzhou, P. R. China;(2) Present address: College of Life Science, Zhejiang Forestry University, 311300 Lin An, P. R. China;
Abstract:Upland and lowland rice (Oryza sativa L.) showed different mechanisms of water stress resistance. Hydroponically grown 3-week-old seedlings of a lowland variety IR64 and an upland variety were exposed to 15% polyethylene glycol (PEG-6000). After 7 d of treatment, IR64 maintained high relative water content and developed a well-branched root. Therefore, IR64 had better water-deficit tolerance than Azucena under PEG treatment. To identify water-deficit-responsive proteins associated with the tolerance differences between two ecotypes, a comparative proteomic analysis of roots was conducted. Out of 700 proteins reproducibly detected on two-dimensional electrophoresis gels, 65 proteins exhibited significant changes in at least one ecotype at 48 h of water deficit. Only 15 proteins showed different responses to water deficit between the two ecotypes. Twelve proteins were identified by matrix-assisted laser desorption/ionization-time of flight/time of flight-mass spectrometry, which involved in energy and metabolism, protein processing and degradation, detoxification and pathogenrelated (PR) proteins, i.e. PR-1a, RSOsPR10 and JIOsPR10. All three PR proteins were induced more strongly in IR64 than in Azucena by water deficit at both protein and mRNA level. The results suggested that PR-1a, RSOsPR10 and JIOsPR10 may play important roles in protecting root cells against water deficit in rice.
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