Low concentrations of dimethyl sulphoxide accelerate conjugation and incorporation of glycine and glucosamine intetrahymena |
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Authors: | J. E. Richmond |
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Affiliation: | (1) Department of Nutritional Sciences, University of California, 119 Morgan Hall, 94720 Berkeley, CA, USA |
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Abstract: | Summary Dimethyl sulphoxide at relatively low comentrations, 0.01 to 1 mM, enhanced the conjugation and cell-to-cell adhesion of complementary strains of matingTetrahymena thermophila. The time required to form stable conjugates was reduced by dimethyl sulphoxide. This chemical stimulated the uptake of glycine and glucosamine from the suspending media. Incorporation of 2-14C-glycine and 6-3H-D-glucosamine into protein and glycoprotein was enhanced in whole cells, surface membrane and cilia. Incorporation of glucosamine into the microsomal fraction was increased in the dimethyl sulphoxidetreated cells while there was little change in glycine incorporation. There were no detectable changes in glycine and glucosamine incorporation into the nuclear fractions isolated from conjugatingTetrahymena exposed to dimethyl sulphoxide. |
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Keywords: | Tetrahymena Conjugation Cell contacts Mating Dimethyl sulphoxide Protein synthesis Glycoprotein synthesis Surface plasma membrane Pairing Cilia Costimulation Pellicle Surface cell membrane |
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