Paludibacter jiangxiensis sp. nov., a strictly anaerobic,propionate-producing bacterium isolated from rice paddy field |
| |
Authors: | Yan-Ling Qiu Xiao-Zhu Kuang Xiao-Shuang Shi Xian-Zheng Yuan Rong-Bo Guo |
| |
Institution: | 1. Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, No. 189 Songling Road, Qingdao, 266101, Shandong Province, People’s Republic of China 2. GlaxoSmithKline Biologicals (Shenzhen) Co., Ltd, Shenzhen, People’s Republic of China
|
| |
Abstract: | A mesophilic, obligately anaerobic, propionate-producing fermentative bacterium, designated strain NM7T, was isolated from rural rice paddy field. Cells of strain NM7T are Gram-negative, non-motile, non-spore-forming, short rods, and negative for catalase. The strain grew optimally at 37 °C (the range for growth 15–40 °C) and pH 7.0 (pH 5.0–7.5). The strain could grow fermentatively on various sugars, including arabinose, xylose, fructose, galactose, glucose, mannose, cellobiose, lactose, maltose, sucrose, pectin and starch. The main end products of glucose fermentation were acetate and propionate. Yeast extract was not required but stimulated the growth. Nitrate, sulfate, thiosulfate, elemental sulfur, sulfite, and Fe(III) nitrilotriacetate were not used as terminal electron acceptors. The G+C content of genomic DNA was 42.8 mol%. The major cellular fatty acids were C15:0, anteiso-C15:0, C16:0, and C17:0. The most abundant polar lipid of strain NM7T was phosphatidylethanolamine. 16S rRNA gene sequence analysis revealed that it belongs to the family Porphyromonadaceae of the phylum Bacteroidetes. The closest recognized species was Paludibacter propionicigenes (91.4 % similarity in 16S rRNA gene sequence). A novel species, Paludibacter jiangxiensis sp. nov., is proposed to accommodate strain NM7T (=JCM 17480T = CGMCC 1.5150T = KCTC 5844T). |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|