Chloroplast DNA typing by PCR-SSCP in thePinus pumila-P. parviflora var.pentaphylla complex (Pinaceae) |
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Authors: | Yasuyuki Watano Michio Imazu Tatemi Shimizu |
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Institution: | (1) Department of Biology, Faculty of Science, Kanazawa University, 920-11 Kanazawa, Japan;(2) Present address: Institute of Agriculture and Forestry, University of Tsukuba, 305 Tsukuba, Japan |
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Abstract: | Pinus hakkodensis has been considered as a hybrid betweenP. pumila andP. parviflora var.pentaphylla. Chloroplast DNA typing of this putative hybrid and hypothesized parental species in the Tanigawa Mountains, Japan, was conducted
by PCR and single-strand conformation polymorphism (SSCP) of the intergenic spacer betweentrnL(UAA)3′ exon andtrnF(GAA) of cpDNA. Each of the hypothesized parental species collected from other mountain regions displayed a diagnostic SSCP
pattern, whereas all morphological intermediates sampled in the Tanigawa Mountains had the SSCP pattern ofP. parviflora var.pentaphylla. Furthermore, some individuals classified on the basis of needle morphology as belonging toP. pumila in this mountain region showed the SSCP pattern ofP. parviflora var.pentaphylla. This may suggest that pollen-mediated uni-directional introgression fromP. parviflora var.pentaphylla toP. pumila occurs in the Tanigawa Mountains. |
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Keywords: | Chloroplast DNA Cytoplasmic introgression Hybridization Pinus SSCP |
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