MTT方法的优化

目的：对MTT法检测悬浮细胞增殖活性的实验条件进行筛选。方法：以K562细胞为实验对象，分别测定不同MTT用量、细胞浓度、MTT溶剂种类及作用时间等实验条件下的OD570值。结果：检测K562细胞的增殖活性时，细胞浓度应选取0．8×108～0．2×108/L，MTT加入量不应超过20μL/孔，若不考虑时间成本，应以三联溶液作为甲硂溶剂，反应12 h后检测，所获结果精密度最高；若需快速获得结果，也可选择DMSO作为甲硂溶剂，反应10 min后检测。结论：建立了优化的MTT法检测悬浮细胞增殖活性。

The Optimization of MTT Method

Objective：To determine the experimental conditions of MTT method detecting suspension cell proliferation activity.Methods：We took K562 cells as experimental subjects and detected the OD570 value under different experi-mental conditions respectively,including different dosage of MTT,cell concentration,MTT solvent type and reaction time.Results：To detect the proliferation activity of K562 cells,cell concentration should be 0.8×108 ~0.2 ×108/L, the adding amount of MTT should not exceed 20 μL/hole.As time consuming as it was （12 hours after reaction）,the triple-solution was the best formazen solvent for obtaining results with the highest precision,or DMSO could also be a good choice for providing results most quickly （10 minutes after reaction）.Conclusion：We established optimization ex-perimental conditions to detect suspension cell proliferation activity by MTT method.