| 固相萃取-高效液相色谱法测定复合薯片中丙烯酰胺 |
| |
| 引用本文： | 蔡文,谭兴和,张喻,王锋,张春艳,邓洁红.固相萃取-高效液相色谱法测定复合薯片中丙烯酰胺[J].激光生物学报,2014,23(2):178-182. |
| |
| 作者姓名： | 蔡文 谭兴和 张喻 王锋 张春艳 邓洁红 |
| |
| 作者单位： | 柑桔资源综合利用国家地方联合工程实验室;湖南农业大学食品科技学院; |
| |
| 基金项目： | 国家自然科学基金项目(31371817);2012年湖南省研究生科研创新项目(CX2012B314);柑桔加工副产物高效转化关键技术研究及产业化示范(2012BAD31B02) |
| |
| 摘 要： | 建立了一种利用固相萃取小柱与高效液相色谱联用，采用光电二极管阵列检测器测定复合薯片中丙烯酰胺的方法。以0．1％的甲酸水溶液为提取试剂，采用Waters Oasis HLB固相萃取小柱（200mg／6cc）对提取液净化处理，然后以流动相为甲醇／水（5：95，V：V），流速为0．6mL／min，检测波长为210nm，利用AgilentC18反相色谱柱进样分析。在该条件下丙烯酰胺的回收率达80％以上，最低检测限小于10ng／mL。该方法操作简便、重复性好、稳定性高，可用于油炸复合薯片中丙烯酰胺的快速检测。
|
| 关 键 词： | 固相萃取 高效液相色谱 薯片 丙烯酰胺 测定 |
Determination of Acrylamide in Compound Potato Chips Using Solid Phase Extraction and High Performation Liquid Chromatography |
| |
| Institution: | CA1 Wen, TAN Xinghe , ZHANG Yu, WANG Feng, ZHANG Chunyan , DENG Jtehong (1. College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, Hunan, China; 2. National and Local Joint Engineering Laboratory of Citrus Comprehensive Utilization, Changsha 410128, Hunan, China) |
| |
| Abstract: | In order to develop a method for the determination of acrylamide in chips using photodiode array detector with solid phase extraction （SPE） and high performance liquid chromatography （HPLC）, the sample was extracted with 0.1% formic acid in water, and cleaned up by Waters Oasis HLB SPE cartridge （200 mg/3cc） , then used methanol/water（ 5： 95, V/V）as mobile phase at a flow rate of 0.6 mL/min and a wavelength of 210 nm, analyzed by Agilent C ls reverse phase column. The results showed that the average recovery was over 80% ,the limit of detection was less than 10 ng/mL. This method was operated simply with good repeatability and high stability, it could be used for rapid determination of acrylamiade in compound potato chips. |
| |
| Keywords: | solid phase extraction high performance liquid chromatography chips acrylamide determination |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
