A Whole-Plant Microtiter Plate Assay for Drought Stress Tolerance-Inducing Effects |
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Authors: | Torsten Geissler Ludger A Wessjohann |
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Institution: | (1) Department of Bioorganic Chemistry, Leibniz Institute of Plant Biochemistry, Weinberg 3, 06120 Halle (Saale), Germany; |
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Abstract: | The frequency and intensity of extreme weather events and global temperature are rising, which poses a potential threat to
life, specifically crops, and therefore food and bioenergy supply. Reduced water availability has the most severe impact on
potential grain yield. Negative effects of transient drought stress (dry spells) can be countered by drought tolerance-inducing
chemicals. In search for useful compounds, biochemical assays are fast but limited in scope, whereas whole-plant assays are
slow, require large amounts of compounds, and are usually not concentration-related. Here we report the development of a fast,
concentration-dependent whole-plant assay using the fast growing duckweed Lemna minor L. 4-Amino-1,8-naphthalimide (1) and the imidacloprid metabolite 6-chloronicotinic acid (2) were affirmed as drought stress tolerance enhancers. Both also reduce oxidative stress-induced cell death in Arabidopsis thaliana (L.) Heynh. cell suspension culture but show differences in their mode of action. |
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