青阳参组织培养及愈伤组织的成分分析

用青阳参(Cynanchum otophyllum)的嫩枝和芽在Ms 2．0mg／L2，4-D 0．1mg／L KIN的培养基上诱导愈伤组织。通过不同的培养基和激素配比实验，发现6，7-V 2．0mg／L2，4．D 0．3mg／LKIN最适合愈伤组织的生长。但在6，7-V 1．0mg／L2，4．D 0．1mg／L KIN培养基中的愈伤组织次生代谢物含量最高。愈伤组织的生长周期为27d，但在33d时次生代谢产物的含量最高。从愈伤组织中分离到7个化合物：(1)9，10，11-三羟基-十八碳-12(Z)-烯酸甲酯(methyl9，10，11-trihydroxy-12-octadecencate)，(2)胡萝卜甙(daucosterol)，(3)β-谷甾醇(β-sitoster01)，(4)华木酸(betuliniic acid)，(5)齐端果酸(oleamlic acid)，(6)棕榈酸(hexadecanoic acid)，(7)十八碳-9-烯酸(9-octadecenoic acid)。首次报道从植物愈伤组织中分离到多羟基十八碳烯酸，并讨论了化合物（1）对植物细胞生长的可能影响。

Studies on the Tissue Culture of Cynanchum otophyllum and Calli Chemical Constituents

The calli of Cynanchum otophyllum were induced from the tender stems and young buds on MS medium with the supplement of 2.0 mg/L 2,4-D and 0.1 mg/L KIN. The result showed that 6,7-V medium was the optimal medium for callus growth. The tests using plant growth regulators with various concentrations showed that 6,7-V medium plus 2.0 mg/L 2,4-D and 0.3 mg/L KIN was the best combination for callus growth, whereas the production of secondary metabolites was higher on 6,7-V medium plus 1.0 mg/L 2,4-D and 0.1 mg/L KIN. The best period of culture was 27 d for callus growth, and 33 d for the maximal production of secondary metabolites. The isolation of nature products from the callus provided seven compounds including (1) fatty acid methyl ester named methyl 9,10,11-trihydroxy-12-octadecenoate and (2) daucosterol, (3) β-sitosterol, (4) betulinic acid, (5) oleanolic acid, (6) hexadecanoic acid, (7) 9-octadecenoic acid. This is the first report of the isolation of poly-hydroxyl octadecenoic acid from a callus, and its proven biological function on the growth of plant cells was discussed.