Genotype screening for proliferative embryogenesis and biolistic transformation of short-season soybean genotypes |
| |
Authors: | D H Simmonds P A Donaldson |
| |
Institution: | (1) Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Bldg. 21, Central Experimental Farm, Ottawa, Ontario, Canada K1A OC6 e-mail: simmondsdh@em.agr.ca Fax: +613-7596566, CA |
| |
Abstract: | Eighteen of 20 short-season soybean (Glycine max (L.) Merrill) genotypes (maturity group 0 and 00) screened for proliferative embryogenic capacity formed secondary globular
embryos, at rates of 1–70% of cultured immature cotyledons. Five genotypes produced embryogenic cultures which were proliferative
for at least 6 months. Proliferative embryogenic cultures of AC Colibri and X2650–7–2–3 were bombarded using a Bio-Rad PDS-1000/He
particle gun. Co-bombardments with plasmid pairs pHygr (encoding a type IV aminoglycoside phosphotransferase;aphIV) and pRD300pat (encoding a phosphinothricin N-acetyltransferase;pat) or pRD300pat and pFF19G (β-glucuronidase;uidA or gus) resulted, respectively, in 12 hygromycin-selected lines with multiple insertions of aphIV and pat, and two l-phosphinothricin-selected lines plus three β-glucuronidase-positive lines recovered without selection. Although
fertile plants were recovered from young proliferative cultures, transgenic plants, which were derived from cultures 12–14
months of age, were sterile.
Received: 8 January 1998 / Revision received: 12 January 1999 / Accepted: 12 July 1999 |
| |
Keywords: | Soybean Proliferative Embryogenic Bombardment Transformation |
本文献已被 SpringerLink 等数据库收录! |
|