Primary antibody response studied by using inhibitors of nucleic acid synthesis |
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Authors: | Lydie Jarošková J Městecký J Šterzl |
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Institution: | (1) Department of Immunology, Institute of Microbiology, Czechoslovak Academy of Sciences, Prague 4 |
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Abstract: | Inhibitors of nucleic acid synthesis were tested for the effect on the primary antibody response. Antibody formation by adult
spleen cells mixed with antigenin vitro and transferred to newborn rabbits was completely suppressed by 6-mercaptopurine administered in the initial phase of antibody
induction. The extent of inhibition decreased proportionately to the time interval between cell transfer and treatment with
6-MP. Different sensitivity of immunologically competent cells towards 6-MP at various periods of differentiation and interference
of 6-MP with synthesis of specific ribonucleic acid is suggested. Actinomycin D did not show any inhibitory effect on the
primary antibody responsein vivo which may be due either to the toxicity of the drug or to some other mechanism of synthesis of immunoglobulins than is known
for bacterial proteins. Mitomycin C was also ineffective in suppressing the inductive phase of antibody formation. From results
with 6-MP and mitomycin C it is concluded that DNA synthesis and mitotic division are not essential steps in the induction
of antibodies. |
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