Genome mining approach for the discovery of novel cytochrome P450 biocatalysts |
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Authors: | Toshiki Furuya Kuniki Kino |
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Institution: | (1) Department of Applied Chemistry, Faculty of Science and Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan; |
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Abstract: | Cytochrome P450 enzymes (P450s) are able to regioselectively and stereoselectively introduce oxygen into organic compounds
under mild reaction conditions. These monooxygenases in particular easily catalyze the insertion of oxygen into less reactive
carbon–hydrogen bonds. Hence, P450s are of considerable interest as oxidation biocatalysts. To date, although several P450s
have been discovered through screening of microorganisms and have been further genetically engineered, the substrate range
of these biocatalysts is still limited to fulfill the requirements for a large number of oxidation processes. On the other
hand, the recent rapid expansion in the number of reported microbial genome sequences has revealed the presence of an unexpectedly
vast number of P450 genes. This large pool of naturally evolved P450s has attracted much attention as a resource for new oxidation
biocatalysts. In this review, we focus on aspects of the genome mining approach that are relevant for the discovery of novel
P450 biocatalysts. This approach opens up possibilities for exploitation of the catalytic potential of P450s for the preparation
of a large choice of oxidation biocatalysts with a variety of substrate specificities. |
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Keywords: | |
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