Fitness aspects of transgenic <Emphasis Type="Italic">Aedes fluviatilis</Emphasis> mosquitoes expressing a <Emphasis Type="Italic">Plasmodium</Emphasis>-blocking molecule |
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Authors: | Maíra N Santos Paula M Nogueira Fernando B S Dias Denise Valle Luciano A Moreira |
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Institution: | 1.Malaria Laboratory,Centro de Pesquisas René Rachou (CPqRR/FIOCRUZ),Barro Preto, Belo Horizonte,Brazil;2.Laboratory of Triatomines and Epidemiology of Chagas Disease,CpqRR/FIOCRUZ,Barro Preto, Belo Horizonte,Brazil;3.Laboratory of Physiology and Control of Arthropod Vectors,Instituto Oswaldo Cruz (IOC/FIOCRUZ),Manguinhos, Rio de Janeiro,Brazil |
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Abstract: | Vector-born diseases cause millions of deaths every year globally. Alternatives for the control of diseases such as malaria
and dengue fever are urgently needed and the use of transgenic mosquitoes that block parasite/virus is a sound strategy to
be used within control programs. However, prior to use transgenic mosquitoes as control tools, it is important to study their
fitness since different biological aspects might influence their ability to disseminate and compete with wild populations.
We previously reported the construction of four transgenic Aedes fluviatilis mosquito lines expressing a Plasmodium- blocking molecule (mutated bee venom phospholipase A2–mPLA2). Presently we studied two aspects of their fitness: body size, that has been used as a fitness-related status, and the expression
of major enzymes classes involved in the metabolism of xenobiotics, including insecticides. Body size analysis (recorded by
geometric wing morphometrics) indicated that both male and female mosquitoes were larger than the non-transgenic counterparts,
suggesting that this characteristic might have an impact on their overall fitness. By contrast, no significant difference
in the activity of enzymes related to metabolic insecticide resistance was detected in transgenic mosquitoes. The implication
on fitness advantage of these features, towards the implementation of this strategy, is further discussed. |
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