A signal amplification assay for HSV type 1 viral DNA detection using nanoparticles and direct acoustic profiling |
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| Authors: | Yildiz Uluda? Richard Hammond and Matthew A Cooper |
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| Institution: | (1) Cranfield Health, Cranfield University, MK43 0AL Cranfield, Bedfordshire, UK;(2) Cambridge Medical Innovations, 181 Cambridge Science Park, CB4 0GJ Cambridge, UK;(3) Institute for Molecular Bioscience, University of Queensland, 306 Carmody Rd, 4072 St Lucia, Qld, Australia |
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| Abstract: | Background Nucleic acid based recognition of viral sequences can be used together with label-free biosensors to provide rapid, accurate
confirmation of viral infection. To enhance detection sensitivity, gold nanoparticles can be employed with mass-sensitive
acoustic biosensors (such as a quartz crystal microbalance) by either hybridising nanoparticle-oligonucleotide conjugates
to complimentary surface-immobilised ssDNA probes on the sensor, or by using biotin-tagged target oligonucleotides bound to
avidin-modified nanoparticles on the sensor. We have evaluated and refined these signal amplification assays for the detection
from specific DNA sequences of Herpes Simplex Virus (HSV) type 1 and defined detection limits with a 16.5 MHz fundamental
frequency thickness shear mode acoustic biosensor. |
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