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New monoterpene glycosides and phenolic compounds from Distylium racemosum and their inhibitory activity against ribonuclease H |
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| Authors: | Kim Jeong Ah Yang Seo Young Wamiru Anthony McMahon James B Le Grice Stuart F J Beutler John A Kim Young Ho |
| Institution: | a College of Pharmacy, Chungnam National University, Daejeon 305-764, Republic of Korea b Molecular Targets Laboratory, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA c SAIC-Frederick, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA d HIV Drug Resistance Program, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA |
| Abstract: | Two new monoterpene glycosides, distyloside A-B (1-2), and a new megastigmane glucoside, iso-dihydrodendranthemoside A (3) were isolated from twigs and leaves of Distylium racemosum, along with five known phenolic compounds (4-8). The structures were established via spectroscopic techniques and chemical transformations, and the absolute stereochemistry of 3 was determined by Mosher’s esterification. A homogeneous fluorescence resonance energy transfer (FRET) quenching assay was used to determine the inhibitory activity of isolates (1-8) on the ribonuclease H enzymes from HIV-1, 2, human, and Escherichia coli. Among them, 6″-O-galloylsalidroside (6) showed potent inhibitory effects with an IC50 value of 3.5 μM on HIV-2, and 1.7 μM on human RNase H, respectively. |
| Keywords: | Distylium racemosum Monoterpene glycosides Megastigmane glucoside Ribonuclease H |
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