Specificity of receptors to vasoactive intestinal peptide in guinea pig brain. |
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| Authors: | Patrick Robberecht Wolfgang König Monique Deschodt-Lanckman Philippe De Neef Jean Christophe |
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| Affiliation: | 1. Department of Biochemistry and Nutrition, School of Medicine Université libre de Bruxelles, Boulevard de Waterloo 115, B-1000 Brussels, Belgium |
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| Abstract: | The specific binding of VIP to guinea pig brain membranes was tested by 1/ the ability of eight VIP and secretin analogs and fragments to inhibit the binding of 125I-VIP and 2/ the capacity of the same peptides to influence basal and VIP-stimulated adenylate cyclase activities. Among all peptides tested, only VIP, secretin, [Val5] secretin, and [Gln9, Asn15] secretin (5–27) were able to inhibit 125I-VIP binding. The adenylate cyclase activity was stimulated by VIP, secretin and [Val5] secretin. [Gln9, Asn15] secretin (5–27) although inactive per se was able to inhibit the VIP-stimulated adenylate cyclase activity competitively. |
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