| 槲皮素填充的脂质体对电离辐射处理的大鼠胸主动脉平滑肌细胞上BK_(Ca)的作用(英文) |
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| 作者姓名： | SOLOVIEV Anatoly TISHKIN Sergey KYRYCHENKO Sergey |
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| 作者单位： | 乌克兰医学科学院药理和毒理学研究所; |
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| 基金项目： | supported by ‘Centre of Excellence Award Scheme’ (2007-2008) of the Physiological Society |
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| 摘 要： | 本文旨在研究槲皮素填充的卵磷脂脂质体(quercetin-filled phosphatidylcholine liposomes,PCL-Q)对非致死性全身电离辐射处理的大鼠胸主动脉平滑肌细胞(smooth muscle cells,SMCs)上的大电导钙离子依赖性钾通道(large conductance Ca2+-dependent K+channels,BKCa)的作用。我们使用膜片钳技术,观察到去极化阶跃脉冲刺激的SMCs上出现外向K+电流,该电流对paxilline(BKCa的一种特异性抑制剂)敏感,因此认为该电流主体为BKCa电流。相对正常对照大鼠,辐照处理大鼠SMCs的BKCa电流幅度明显减小。这种通道抑制效应在辐照后第9天表现显著,且在整个30天的实验期间是逐渐增强的。辐照处理大鼠的SMCs的血管舒张能力可能因此而减弱。在本实验中,PCL-Q有效地恢复了辐照后SMCs的BKCa功能。而PCL-Q的组分,单一的槲皮素或空白的脂质体(PCL)恢复BKCa功能的能力较二者的组合体有明显的减弱。上述结果表明,PCL-Q能恢复辐照处理后SMCs的BKCa正常功能。PCL-Q的保护能力可能来源于其抗氧化...
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| 关 键 词： | 平滑肌 辐射 活性氧 钾通道 槲皮素 脂质体 蛋白激酶C |
Quercetin-filled phosphatidylcholine liposomes restore abnormalities in rat thoracic aorta BK_(Ca) channel function following ionizing irradiation |
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| SOLOVIEV Anatoly,TISHKIN Sergey,KYRYCHENKO Sergey.Quercetin-filled phosphatidylcholine liposomes restore abnormalities in rat thoracic aorta BK_(Ca) channel function following ionizing irradiation[J].Acta Physiologica Sinica,2009(3). |
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| Authors: | SOLOVIEV Anatoly TISHKIN Sergey KYRYCHENKO Sergey Institute of Pharmacology Toxicology Academy of Medical Science Kiev Ukraine |
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| Institution: | SOLOVIEV Anatoly,TISHKIN Sergey,KYRYCHENKO Sergey Institute of Pharmacology and Toxicology,Academy of Medical Science,Kiev 03057,Ukraine |
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| Abstract: | The goal of the present study was to investigate the effects of quercetin-filled phosphatidylcholine liposomes(PCL-Q) on the currents carried by large conductance Ca2+-dependent K+ channels(BKCa) in rat thoracic aorta following non-fatal whole-body ionizing irradiation. Using patch-clamp technique,it is found that the outward K+ currents of isolated smooth muscle cells(SMCs) stimulated by depolarizing voltage steps were sensitive to BKCa inhibitor,paxilline,and this kind of outward K+ currents in SMCs from ... |
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| Keywords: | smooth muscle radiation reactive oxygen species potassium channels quercetin liposomes protein kinase C |
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