| mRNA差异展示研究胃癌差异表达基因 |
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| 引用本文： | 崔大祥,闫小君,王枫,赵锦荣,苏成芝.mRNA差异展示研究胃癌差异表达基因[J].生物化学与生物物理进展,2000,27(4):379-382. |
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| 作者姓名： | 崔大祥 闫小君 王枫 赵锦荣 苏成芝 |
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| 作者单位： | 第四军医大学全军基因诊断技术研究所,西安,710033 |
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| 基金项目： | 陕西省卫生厅重点基金资助项目（99ZH-002）. |
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| 摘 要： | 以胃癌细胞株GC7901与胃粘膜细胞株GES-1为对比材料，利用mRNA差异展示技术，研究胃粘膜至胃癌过程的差异表达基因，为建立胃癌预警系统打下基础.获得8个未知的与胃癌相关的cDNA,命名为GCYS-1，2，3，4，5，6，7，20，完成其克隆及序列分析，RNA印迹证实GCYS-1至7片段与GCYS-20基因在GC7901细胞株中呈高表达，在GES-1细胞株中呈低表达.此8个序列在GenBank数据库中登录，接受号为AF054162～AF056168及AF219140.
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| 关 键 词： | 胃癌，细胞株，mRNA差异展示，基因克隆，核苷酸序列分析 |
| 收稿时间： | 1999/6/28 0:00:00 |
| 修稿时间： | : |
Studies on Differentially Expressed Genes of Gastric Cancer by mRNA Differential Display |
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| CUI Da-Xiang,YAN Xiao-Jun,WANG Feng,ZHAO Jin-Rong and SU Cheng-Zhi.Studies on Differentially Expressed Genes of Gastric Cancer by mRNA Differential Display[J].Progress In Biochemistry and Biophysics,2000,27(4):379-382. |
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| Authors: | CUI Da-Xiang YAN Xiao-Jun WANG Feng ZHAO Jin-Rong and SU Cheng-Zhi |
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| Abstract: | In order to establish warning system of gastric cancer, using cell line GC7901 and GES-1 as targets,8 cDNAs of differentially expressed genes between them were isolated by mRNA differential display,and then respectively named as GCYS-1 to GCYS-7,GCYS-20,and then were cloned into pGEM-T vector. These cDNA fragments were up-regulated in GC7901 and down-regulated in GES-1 as shown by Northern blot.DNA sequencing demonstrated that all of them were novel.These sequences have been assigned the database accession numbers in GenBank as below:AF054162,AF054163,AF054164,AF054165,AF054166,AF054167,AF054168,AF219140. |
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| Keywords: | gastric cancer cell line mRNA differential display gene cloning sequencing of DNA |
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