The demonstration of adenosinetriphosphatase activity with the electronmicroscope |
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| Authors: | J -P Persijn W Th Daems J C H de Man A E F H Meijer |
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| Institution: | (1) From the Laboratory for Electronmicroscopy, University of Leiden, The Netherlands;(2) Laboratory for Pathology, University of Leiden, The Netherlands |
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| Abstract: | Summary A new method for the demonstration of adenosine triphosphatase (ATP'ase) activity in tissue blocks with the electron-microscope has been developed. This method consists of short fixation (2–3 minutes,ph 7.3) in a OsO4-Tris-HCl mixture, incubation with a medium containing Tris-HCl buffer, tartrate, lead and magnesium ions, ATP and sucrose (ph 6.8) and routine postfixation. The method combines satisfactory histochemical specificity with good preservation of tissue fine structure. Estimations of enzymatic activity of ATP'ase and alkaline phosphatase, as observed with the electronmicroscope, were compared with activity determinations in tissue homogenates. The occurence of a positive histochemical reaction in swollen mitochondria is discussed.With 9 Figures in the Text |
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