Permeabilization ofNeurospora crassa hyphae with toluene-ethanol and filipin |
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Authors: | David R. Quigley Evelyn Jabri Dr. Claude P. Selitrennikoff |
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Affiliation: | (1) Department of Cellular and Structural Biology, University of Colorado Health Sciences Center, 4200 Easth Ninth Avenue, 80262 Denver, Colorado, USA |
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Abstract: | Young hyphae ofNeurospora crassa were made permeable to UDP-glucose and trypan blue by treatment with toluene-ethanol and filipin. Less than 2% of treated cells survived treatment with 8% and 16% toluene-ethanol, while 25% survived treatment with 4% toluene-ethanol. Similarly, 98% of treated cells were killed by treatment with 16 g/ml filipin. Electron microscopy revealed that toluene-ethanol-treated cells lost pieces of plasma membrane and contained a number of vacuole-like structures; filipin-treated cells were less affected. Both filipin- and toluene-ethanol-treated cells were able to incorporate UDP-glucose into insoluble material (likely glycogen and glucan). |
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