Dexamethasone induces increased catecholamine biosynthesis in cultured neuroblastoma |
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Authors: | D. Sandquist A. C. Black Jr S. K. Sahu L. Williams T. H. Williams |
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Affiliation: | Department of Microbiology and Cell Biology, The Pennsylvania State University, University Park, PA 16802, USA |
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Abstract: | The effect of dexamethasone on morphological and biochemical parameters of catecholamine biosynthesis in cultured murine neuroblastoma cells was determined. Treatment for 7 days with 25 μM dexamethasone produced a more intense and uniform catecholamine fluorescence as determined by fluorescence histochemistry using the glyoxylic acid technique. Treatment with dexamethasone also produced a two-fold increase in dopamine content and a threefold increase in tyrosine hydroxylase activity compared with controls. These results suggest that this potent glucocorticoid induces differentiation in cultured murine neuroblastoma, as measured by both biochemical and morphological techniques. |
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Keywords: | To whom reprint requests should be addressed. |
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