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炭疽杆菌芽孢外壁胶原样蛋白(BclA)的多态性分析
引用本文:李冠霖,徐俊杰,董大勇,宋小红,陈薇.炭疽杆菌芽孢外壁胶原样蛋白(BclA)的多态性分析[J].微生物学通报,2006,33(1):100-104.
作者姓名:李冠霖  徐俊杰  董大勇  宋小红  陈薇
作者单位:军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京,100071
基金项目:国家自然科学基金资助项目(No.30300016)
摘    要:炭疽杆菌芽孢外壁胶原样蛋白(BclA)是芽孢外壁发状菌丝的主要结构成分,也是芽孢的主要免疫原。从国内分离的3株炭疽杆菌中克隆出BclA基因并进行了序列分析,结果发现有2株(A16R和40048)的BclA与国外报道菌株长度不同,分别含有388个和322个氨基酸,72个和50个GXX三氨基酸重复序列,5个和3个含21个氨基酸的(GPT)5 GDTGTT重复序列(BclA重复)。另一株40022的BclA与国外报道的53169株完全一敛,含有370个氨基酸,66个GXX重复,5个BclA重复。对我国炭疽杆菌BclA蛋白多态性的分析为进行炭疽杆菌的基因分型以及研究炭疽芽孢的免疫原性和致病机理打下基础。

关 键 词:炭疽杆菌  BclA蛋白  多态性  芽孢
文章编号:0253-2654(2006)01-0100-05
收稿时间:2005-04-25
修稿时间:2005-06-04

Polymorphism of the Bacillus anthracis BclA Protein
LI Guan-Lin,XU Jun-Jie,DONG Da-Yong,SONG Xiao-Hong,CHEN Wei.Polymorphism of the Bacillus anthracis BclA Protein[J].Microbiology,2006,33(1):100-104.
Authors:LI Guan-Lin  XU Jun-Jie  DONG Da-Yong  SONG Xiao-Hong  CHEN Wei
Institution:State Key laboratory of Pathogen and Biosafety, Beijing Institute of Microbiology and Epidemiology, Beijing 100071
Abstract:Bacillus anthracis collagen-like protein(BclA) is a structural component of the exosporium filaments,as well as the immunodominant antigen on the spore surface.The genes encoding BclA proteins were cloned and sequenced from three Bacillus anthracis strains separated from China.It was founded that the BclA proteins of strain A16R and 40048,containing 388 and 322 amino acids,72 and 50 copies of GXX repeat,5 and 3 copies of 21-amino-acid sequence(GPT)_(5)GDTGTT(BclA repeat) respectively,are different from those reported by foreign scholars;while the BclA protein of strain 40022,containing 370 amino acids,66 copies of GXX repeat,and 5 copies of BclA repeat,is identical with that of strain 53169 reported by others.The results are helpful for the molecular typing of B.anthracis strains,and provide a basis for the elucidation of the pathogenesis and immunogenicity of B.anthracis spore.
Keywords:Bacillus anthracis  BclA protein  Polymorphism  Spore
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